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alexa fluor 488 monoclonal antibody labeling kit  (Thermo Fisher)


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    Thermo Fisher alexa fluor 488 monoclonal antibody labeling kit
    Alexa Fluor 488 Monoclonal Antibody Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+antibody+labeling+kit/alexa+fluor+488+protein+labeling+kit/pmc12281710-154-0-9
    Average 90 stars, based on 1 article reviews
    alexa fluor 488 monoclonal antibody labeling kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Method of analyzing image of cell in laminated structure and method of evaluating laminated structure for corneal transplantation
    Article Snippet: In fluorescent labeling of AE5, an unlabeled anti-AE5 antibody (manufactured by PROGEN, Product Number: 61807) or an anti-AE5 antibody (Alexa488 labeled anti-AE5 antibody) directly labeled with Alexa488 in advance was used. .. The labeling of Alexa488 to an anti-AE5 antibody was performed using a monoclonal antibody labeling kit (manufactured by Invitrogen, Product Number: A20181). ..

    Article Title: Molecular Mechanism of HER2 Rapid Internalization and Redirected Trafficking Induced by Anti-HER2 Biparatopic Antibody
    Article Snippet: Pitstop 2 (Cat# C7487) was purchased from Cellagen Technology (San Diego, CA, USA). .. Anti-HER2 antibodies (trastuzumab, 39S and anti-HER2-Bs) were labeled with Alexa Fluor 647 (AF647) using Monoclonal Antibody Labeling Kit (Cat# A20186, Life Technologies) according to the manufacturer’s protocol. .. Before labeling, the buffer of the antibodies was replaced with phosphate buffered saline PBS using Zeba Spin Desalting Columns, 40K MWCO (Cat# 87768, Thermo Scientific, Waltham, MA, USA).

    Article Title: Hedgehog pathway responsiveness correlates with the presence of primary cilia on prostate stromal cells
    Article Snippet: The following secondary antibodies were purchased from Molecular Probes and used at 1:200 dilution; Alexa Fluor 546 goat anti- rabbit IgG; Alexa fluor 488 goat anti mouse IgG; Alexa fluor 546 goat anti-mouse IgG. .. Mouse anti-p63 (Santa Cruz, sc-8431, 1:100) antibody was directly labeled with Alexa Fluor 488 using a monoclonal antibody labeling kit (Invitrogen, A-20181). .. Slides were mounted with Vectashield Hardset + DAPI mounting media (Vector, Burlingame, CA) and imaged using an Olympus BX51 or BD pathway fluorescent microscope.

    Article Title: A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor-647 according to the manufacturer’s protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..

    Article Title: Dual antithrombotic therapy dose-dependently alters hemostatic plug structure and function.
    Article Snippet: 1016 jthjournal.org Abstract Background: Antithrombotic medications carry an inherent risk of bleeding, which may be exacerbated when anticoagulant and antiplatelet therapeutics are combined.. Prior studies have shown different effects of antiplatelet vs anticoagulant drugs on the structure and function of hemostatic plugs in vivo.. Objectives: We examined whether dual antithrombotic treatment consisting of combined antiplatelet and anticoagulant therapeutics alters hemostatic plug structure and function differently from treatment with either therapeutic alone.

    Article Title: Antibodies against Mac-1
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor 647 according to the manufacturers protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..

    Antibody Labeling:

    Article Title: Method of analyzing image of cell in laminated structure and method of evaluating laminated structure for corneal transplantation
    Article Snippet: In fluorescent labeling of AE5, an unlabeled anti-AE5 antibody (manufactured by PROGEN, Product Number: 61807) or an anti-AE5 antibody (Alexa488 labeled anti-AE5 antibody) directly labeled with Alexa488 in advance was used. .. The labeling of Alexa488 to an anti-AE5 antibody was performed using a monoclonal antibody labeling kit (manufactured by Invitrogen, Product Number: A20181). ..

    Article Title: Molecular Mechanism of HER2 Rapid Internalization and Redirected Trafficking Induced by Anti-HER2 Biparatopic Antibody
    Article Snippet: Pitstop 2 (Cat# C7487) was purchased from Cellagen Technology (San Diego, CA, USA). .. Anti-HER2 antibodies (trastuzumab, 39S and anti-HER2-Bs) were labeled with Alexa Fluor 647 (AF647) using Monoclonal Antibody Labeling Kit (Cat# A20186, Life Technologies) according to the manufacturer’s protocol. .. Before labeling, the buffer of the antibodies was replaced with phosphate buffered saline PBS using Zeba Spin Desalting Columns, 40K MWCO (Cat# 87768, Thermo Scientific, Waltham, MA, USA).

    Article Title: The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles
    Article Snippet: Alexa 647-conjugated WGA, Alexa 488-conjugated annexin V, Alexa 555-conjugated annexin V, Alexa 647-conjugated annexin V, and Alexa 647-conjugated phalloidin were purchased from Life Technologies. .. For imaging and flow cytometry, recombinant anti-BIN1 exon 13 was conjugated with Alexa 647 using a monoclonal antibody labeling kit (Life Technologies). ..

    Article Title: Hedgehog pathway responsiveness correlates with the presence of primary cilia on prostate stromal cells
    Article Snippet: The following secondary antibodies were purchased from Molecular Probes and used at 1:200 dilution; Alexa Fluor 546 goat anti- rabbit IgG; Alexa fluor 488 goat anti mouse IgG; Alexa fluor 546 goat anti-mouse IgG. .. Mouse anti-p63 (Santa Cruz, sc-8431, 1:100) antibody was directly labeled with Alexa Fluor 488 using a monoclonal antibody labeling kit (Invitrogen, A-20181). .. Slides were mounted with Vectashield Hardset + DAPI mounting media (Vector, Burlingame, CA) and imaged using an Olympus BX51 or BD pathway fluorescent microscope.

    Article Title: Linkage between endosomal escape of LNP-mRNA and loading into EVs for transport to other cells
    Article Snippet: The capture antibody (3F6, MAIIA diagnostics) was biotinylated according to kit insert using EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Scientific). .. The detection antibody (7D3, MAIIA diagnostics) was Alexa 647-labelled using monoclonal antibody labeling kit (Thermo Scientific). .. The hEPO protein (in-house) was used to generate a standard curve in Rexxip A buffer (Gyros Protein Technologies) ranging from 12.2 pg/mL to 50 ng/mL.

    Article Title: A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor-647 according to the manufacturer’s protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..

    Article Title: Dual antithrombotic therapy dose-dependently alters hemostatic plug structure and function.
    Article Snippet: 1016 jthjournal.org Abstract Background: Antithrombotic medications carry an inherent risk of bleeding, which may be exacerbated when anticoagulant and antiplatelet therapeutics are combined.. Prior studies have shown different effects of antiplatelet vs anticoagulant drugs on the structure and function of hemostatic plugs in vivo.. Objectives: We examined whether dual antithrombotic treatment consisting of combined antiplatelet and anticoagulant therapeutics alters hemostatic plug structure and function differently from treatment with either therapeutic alone.

    Article Title: Antibodies against Mac-1
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor 647 according to the manufacturers protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..

    Imaging:

    Article Title: The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles
    Article Snippet: Alexa 647-conjugated WGA, Alexa 488-conjugated annexin V, Alexa 555-conjugated annexin V, Alexa 647-conjugated annexin V, and Alexa 647-conjugated phalloidin were purchased from Life Technologies. .. For imaging and flow cytometry, recombinant anti-BIN1 exon 13 was conjugated with Alexa 647 using a monoclonal antibody labeling kit (Life Technologies). ..

    Flow Cytometry:

    Article Title: The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles
    Article Snippet: Alexa 647-conjugated WGA, Alexa 488-conjugated annexin V, Alexa 555-conjugated annexin V, Alexa 647-conjugated annexin V, and Alexa 647-conjugated phalloidin were purchased from Life Technologies. .. For imaging and flow cytometry, recombinant anti-BIN1 exon 13 was conjugated with Alexa 647 using a monoclonal antibody labeling kit (Life Technologies). ..

    Recombinant:

    Article Title: The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles
    Article Snippet: Alexa 647-conjugated WGA, Alexa 488-conjugated annexin V, Alexa 555-conjugated annexin V, Alexa 647-conjugated annexin V, and Alexa 647-conjugated phalloidin were purchased from Life Technologies. .. For imaging and flow cytometry, recombinant anti-BIN1 exon 13 was conjugated with Alexa 647 using a monoclonal antibody labeling kit (Life Technologies). ..

    Binding Assay:

    Article Title: A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor-647 according to the manufacturer’s protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..

    Article Title: Antibodies against Mac-1
    Article Snippet: .. To test binding of the antibody anti-M7 to human leukocytes, anti-M7 was labeled with Alexa Fluor 647 according to the manufacturers protocols (Monoclonal Antibody Labeling Kit, Life Technologies). ..



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